The effects of Puararia flavonoids(PF) on bovine vascular endothelial cells(VEC) in vitro injured by advanced glycation end products(AGEs) were investigated. When VEC was incubated with AGEs(4 g/L) for 48 hours, the growth of VEC was enhanced, the contents of lactate dehydrogenase(LDH) and malondialdehyde(MDA) and lipofuscin(LPF) were markedly increased(P0.01), the contents of nitric oxide(NO-2/ NO-3) and glutatuhione(GSH) were reduced. Treatment with PF caused a reduction in LDH and MDA and LPF, the contents of GSH and NO-2/ NO-3 were increased. The results suggest AGEs has direct effects on VEC, perhaps oxidative stress participates in this process. PF can protect cultured VEC from being injured by AGEs, thereby exert favorable effect against the occurrence and development of chronic vascular complications in diabetic mellitus.