Ex-treatment of immunoassay method for nodularin and cylindrospermopsins

LI Shuang; YIN Hao-wen

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Nov. 2011

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LI Shuang, YIN Hao-wen. Ex-treatment of immunoassay method for nodularin and cylindrospermopsins[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 108-114.

Citation:

LI Shuang, YIN Hao-wen. Ex-treatment of immunoassay method for nodularin and cylindrospermopsins[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 108-114.

LI Shuang, YIN Hao-wen. Ex-treatment of immunoassay method for nodularin and cylindrospermopsins[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 108-114.

Citation:

LI Shuang, YIN Hao-wen. Ex-treatment of immunoassay method for nodularin and cylindrospermopsins[J]. Journal of East China Normal University (Natural Sciences), 2011, (6): 108-114.

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Ex-treatment of immunoassay method for nodularin and cylindrospermopsins

LI Shuang¹,
YIN Hao-wen²

1.
School of Life Science, East China Normal University, Shanghai 200062, China;
2.
The Bioassay and Safety Assessment,Shanghai Academy of Public Measurement, Shanghai 201203, China

Received Date: 2010-11-01
Rev Recd Date: 2011-02-01
Publish Date: 2011-11-25

Abstract

Abstract

Effective solid phase extraction (SPE) ex-treatment method and sensitive enzyme-linked immunosorbent asssay (ELISA) were developed for determining the nodularins(NOD) and cylindrospermopsins(CYN) in water samples. Due to the different polarities, two different ex-treatment methods were applied to NOD and CYN, respectively. The methods were developed as followed: ① For NOD,firstly the C18 column was activated by 10 mL methanol and 10 mL Milli-Q water; then the sample was applied to the column at 3-4 mL/min; after washing the column with 10 mL water, the NOD was eluted with 10 mL methanol; then the eluent was dried up with a rotary evaporator, and lastly the final volume was fixed to 1 mL with Milli-Q water for ELISA analyse. ② For CYN, the carb SPE tubes were activated with 10mL methanol and 10mL Milli-Q water; then the sample was applied to the tubes at 2-3 mL/min; after washing the tubes with 10mL methanol, the CYN was eluted with 12 mL methanol containing 0.1% trifluoroacetic acid;then the eluent was dried up with a rotary evaporator, lastly the final volume was fixed to 1 mL with Milli-Q water containing 0.1% ammonia for ELISA analyse. The results indicated that these methods can guarantee the good recoveries of NOD and CYN for ELISA analyse, which could reach to 94.3% and 77%, respectively.
- nodularins,
- cylindrospermopsins,
- ELISAs

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References(1)

References

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