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Issue 6
Jan.  2013
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ZHANG Kun-zhi, SU Ming-bo, ZHOU Yu-bo. Establishment of high-throughput-screening model for IRE1 kinase inhibitor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 103-113.
Citation: ZHANG Kun-zhi, SU Ming-bo, ZHOU Yu-bo. Establishment of high-throughput-screening model for IRE1 kinase inhibitor[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 103-113.

Establishment of high-throughput-screening model for IRE1 kinase inhibitor

  • Received Date: 2012-01-01
  • Rev Recd Date: 2012-04-01
  • Publish Date: 2012-11-25
  • This paper expressed and purified the cytosolic fragment of human IRE1 (inositol-requiring enzyme1), an ER transmembrane 'sensor〖KG-*9〗' protein with bacular expression system. Then the HTRF (Homogeneous Time-resolved Fluorescence) assay for IRE1 kinase was set up and optimized. Z-factor is about 0.58 and the coefficients of variation (CV) is less than 10%, indicating the molecue-based high-throughput screening assay for IRE1 kinase inhibitor was successfully established. This work would lay a solid foundation for the discovery of IRE1 kinase inhibitor and the further research.
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