Two strategies for efficient expression of soluble recombinant human FGF-21 protein

LI Juan; LIU Wen; XIAO Lei; LAO Xun; ZHAO Li-fen; HUANG Jing; WU Zi-rong

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2012 > (6): 114-121

LI Juan, LIU Wen, XIAO Lei, LAO Xun, ZHAO Li-fen, HUANG Jing, WU Zi-rong. Two strategies for efficient expression of soluble recombinant human FGF-21 protein[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 114-121.

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LI Juan, LIU Wen, XIAO Lei, LAO Xun, ZHAO Li-fen, HUANG Jing, WU Zi-rong. Two strategies for efficient expression of soluble recombinant human FGF-21 protein[J]. Journal of East China Normal University (Natural Sciences), 2012, (6): 114-121.

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Two strategies for efficient expression of soluble recombinant human FGF-21 protein

1.
School of Life Sciences, East China Normal University, Shanghai 200062， China

Received Date: 2011-09-01
Rev Recd Date: 2011-12-01
Publish Date: 2012-11-25

Abstract

Abstract

The expression of recombinant soluble FGF-21 protein was achieved through transferring another plasmid pTf16 which expressed molecular chaperone Tig. On the other hand, the yield of FGF-21 was greatly improved with the method of lactose auto-induction. The result showed that the soluble TrxA-FGF-21 fusion protein reached 92.4% by molecular chaperone co-expression. With lactose auto-induction, the expression quantity of fusion protein was 17.4% of the total proteins, and the 〖WTBX〗OD600 〖WTBZ〗 was 12.4; thus relative yield was 5.5 times higher than IPTG induction. The biological activities of recombinant FGF-21 were studied 〖WTBX〗in vivo〖WTBZ〗 in healthy C57BL/6 mice. It showed that recombinant FGF-21 can lower blood glucose obviously in mice (〖WTBX〗P〖WTBZ〗 0.05 or 〖WTBX〗P 〖WTBZ〗0.01). The recombinant FGF-21 treatment also led to a significant decrease in concentration of triglyceride in C57BL/6 mice (〖WTBX〗P〖WTBZ〗 0.01).
- fibroblast growth factor 21,
- molecular chaperone,
- lactose auto-induction

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References(1)

References

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