［1］ KURO-O M, MATSUMURA Y, AIZAWA H, et al. Mutation of the mouse klotho gene leads to a syndrome resembling ageing［J］. Nature, 1997, 390(6655): 45-51.

［2］ NAGAI T, YAMADA K, KIM H C, et al. Cognition impairment in the genetic model of aging klotho gene mutant mice: a role of oxidative stress ［J］. Faseb J, 2003, 17(1): 50-52.

［3］ SHIOZAKI M, YOSHIMURA K, SHIBATA M, et al. Morphological and biochemical signs of age-related neurodegenerative changes in klotho mutant mice［J］. Neuroscience, 2008, 152(4): 924-941.

［4］ KUROSU H, YAMAMOTO M, CLARK J D, et al. Suppression of aging in mice by the hormone klotho［J］. Science, 2005, 309(5742): 1829-1833.

［5］ MATSUMURA Y, AIZAWA H, SHIRAKI-IIDA T, et al. Identification of the human klotho gene and its two transcripts encoding membrane and secreted klotho protein［J］. Biochemical and Biophysical Research Communications, 1998, 242(3): 626-630.

［6］ SHIRAKI-IIDA T, AIZAWA H, MATSUMURA Y, et al. Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein［J］. FEBS Letters, 1998, 424(1-2): 6-10.

［7］ URAKAWA I, YAMAZAKI Y, SHIMADA T, et al. Klotho converts canonical FGF receptor into a specific receptor for FGF23［J］. Nature, 2006, 444(7120): 770-774.

［8］ CHANG Q, HOEFS S, VAN DER KEMP A W, et al. The beta-glucuronidase klotho hydrolyzes and activates the TRPV5 channel［J］. Science, 2005, 310(5747): 490-493.

［9］ CHA S K, ORTEGA B, KUROSU H, et al. Removal of sialic acid involving klotho causes cell-surface retention of TRPV5 channel via binding to galectin-1［J］. Proc Natl Acad Sci USA, 2008, 105(28): 9805-9810.

［10］ IMURA A, TSUJI Y, MURATA M, et al. alpha-Klotho as a regulator of calcium homeostasis［J］. Science, 2007, 316(5831): 1615-1618.

［11］ YAMAMOTO M, CLARK J D, PASTOR J V, et al. Regulation of oxidative stress by the anti-aging hormone klotho［J］. Journal of Biological Chemistry, 2005, 280(45): 38029-38034.

［12］ MAEKAWA Y, ISHIKAWA K, YASUDA O, et al. Klotho suppresses TNF-alpha-induced expression of adhesion molecules in the endothelium and attenuates NF-kappaB activation［J］. Endocrine, 2009, 35(3): 341-346.

［13］ LIU F, WU S, REN H, et al. Klotho suppresses RIG-I-mediated senescence-associated inflammation［J］. Nature Cell Biology, 2011, 13(3): 254-262.

［14］ MORENO J A, IZQUIERDO M C, SANCHEZ-NINO M D, et al. The inflammatory cytokines TWEAK and TNFalpha reduce renal klotho expression through NFkappaB［J］. Journal of the American Society of Nephrology, 2011, 22(7): 1315-1325.

［15］ ZHAO Y, BANERJEE S, DEY N, et al. Klotho depletion contributes to increased inflammation in kidney of the db/db mouse model of diabetes via RelA (serine)536 phosphorylation［J］. Diabetes, 2011, 60(7): 1907-1916.

［16］ FENG R, WANG H, WANG J, et al. Forebrain degeneration and ventricle enlargement caused by double knockout of Alzheimer’s presenilin-1 and presenilin-2［J］. Proc Natl Acad Sci USA, 2004, 101(21): 8162-8167.

［17］ SAURA C A, CHOI S Y, BEGLOPOULOS V, et al. Loss of presenilin function causes impairments of memory and synaptic plasticity followed by age-dependent neurodegeneration［J］. Neuron, 2004, 42(1): 23-36.

［18］ BEGLOPOULOS V, SUN X, SAURA C A, et al. Reduced beta-amyloid production and increased inflammatory responses in presenilin conditional knock-out mice［J］. Journal of Biological Chemistry, 2004, 279(45): 46907-46914.

［19］ JIANG X, ZHANG D, SHI J, et al. Increased inflammatory response both in brain and in periphery in presenilin 1 and presenilin 2 conditional double knock-out mice［J］. Journal of Alzheimers Disease, 2009, 18(3): 515-523.

［20］ ZHU M, GU F, SHI J, et al. Increased oxidative stress and astrogliosis responses in conditional double-knockout mice of Alzheimer-like presenilin-1 and presenilin-2［J］. Free Radical Biology and Medicine, 2008, 45(10): 1493-1499.

［21］ WANG D, YANG L, SU J, et al. Attenuation of neurodegenerative phenotypes in Alzheimer-like presenilin 1/presenilin 2 conditional double knockout mice by EUK1001, a promising derivative of xanomeline［J］. Biochemical and Biophysical Research Communications, 2011, 410(2): 229-234.

［22］ DUCE J A, PODVIN S, HOLLANDER W, et al. Gene profile analysis implicates klotho as an important contributor to aging changes in brain white matter of the rhesus monkey［J］. Glia, 2008, 56(1): 106-117.

［23］ YAMAZAKI Y, IMURA A, URAKAWA I, et al. Establishment of sandwich ELISA for soluble alpha-klotho measurement: Age-dependent change of soluble alpha-klotho levels in healthy subjects［J］. Biochemical and Biophysical Research Communications, 2010, 398(3): 513-518.

［24］ KOH N, FUJIMORI T, NISHIGUCHI S, et al. Severely reduced production of klotho in human chronic renal failure kidney［J］. Biochemical and Biophysical Research Communications, 2001, 280(4): 1015-1020.

［25］ HU M C, KURO-O M, MOE O W. Secreted klotho and chronic kidney disease［J］. Advances in Experimental Medicine and Biology, 2012, 728: 126-157.

［26］ SUGIURA H, YOSHIDA T, TSUCHIYA K, et al. Klotho reduces apoptosis in experimental ischaemic acute renal failure［J］. Nephrology Dialysis Transplantation, 2005, 20(12): 2636-2645.

［27］ KOSAKAI A, ITO D, NIHEI Y, et al. Degeneration of mesencephalic dopaminergic neurons in klotho mouse related to vitamin D exposure［J］. Brain Research, 2011, 1382: 109-117.

［28］ LI H, WANG B, WANG Z, et al. Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP［J］. Proc Natl Acad Sci USA, 2010, 107(40): 17362-17367.

［29］ 〖JP+3〗WU P, SHEN Q, DONG S, et al. Calorie restriction ameliorates neurodegenerative phenotypes in forebrain-specific presenilin-1 and presenilin-2 double knockout mice［J］. Neurobiology of Aging, 2008, 29(10): 1502-1511.〖JP〗

［30］ DONG S, LI C, WU P, et al. Environment enrichment rescues the neurodegenerative phenotypes in presenilins-deficient mice［J］. European Journal of Neuroscience, 2007, 26(1): 101-112.

［31］ BERNDT T, KUMAR R. Phosphatonins and the regulation of phosphate homeostasis［J］. Annual Review of Physiology, 2007, 69: 341-359.

［32］  KUROSU H, OGAWA Y, MIYOSHI M, et al. Regulation of fibroblast growth factor-23 signaling by klotho［J］. Journal of Biological Chemistry, 2006, 281(10): 6120-6123.

［33］ TSUJIKAWA H, KUROTAKI Y,FUJIMORI T, et al. Klotho, a gene related to a syndrome resembling human premature aging, functions in a negative regulatory circuit of vitamin D endocrine system［J］. Molecular Endocrinology, 2003, 17(12): 2393-2403.
［34］ BROWNSTEIN C A, ADLER F, NELSON-WILLIAMS C, et al. A translocation causing increased alpha-klotho level results in hypophosphatemic rickets and hyperparathyroidism［J］. Proc Natl Acad Sci USA, 2008, 105(9): 3455-3460.

［35］ KESTENBAUM B, SAMPSON J N, RUDSER K D, et al. Serum phosphate levels and mortality risk among people with chronic kidney disease［J］. Journal of the American Society of Nephrology, 2005, 16(2): 520-528.

［36］ RAKUGI H, MATSUKAWA N, ISHIKAWA K, et al. Anti-oxidative effect of klotho on endothelial cells through cAMP activation［J］. Endocrine, 2007, 31(1): 82-87.

［37］ GU F, ZHU M, SHI J, et al. Enhanced oxidative stress is an early event during development of Alzheimer-like pathologies in presenilin conditional knock-out mice［J］. Neuroscience Letters, 2008, 440(1): 44-48.

［38］ HERREMAN A, HARTMANN D, ANNAERT D, et al. Presenilin 2 deficiency causes a mild pulmonary phenotype and no changes in amyloid precursor protein processing but enhances the embryonic lethal phenotype of presenilin 1 deficiency［J］. Proc Natl Acad Sci USA, 1999, 96(21): 11872-11877.