Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors

WU Jin; LU Xiao-ming; DUAN Ya-le

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Mar. 2013

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WU Jin, LU Xiao-ming, DUAN Ya-le. Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 84-91.

Citation:

WU Jin, LU Xiao-ming, DUAN Ya-le. Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 84-91.

WU Jin, LU Xiao-ming, DUAN Ya-le. Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 84-91.

Citation:

WU Jin, LU Xiao-ming, DUAN Ya-le. Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors[J]. Journal of East China Normal University (Natural Sciences), 2013, (2): 84-91.

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Cyclo (L-Pro-L-Phe) dipeptide: the potential phosphodiesterase inhibitors

1.
Key Laboratory of Brain Functional Genomics, Ministry of Education， Shanghai Key Laboratory of Brain Functional Genomics, East China Normal University, Shanghai 200062, China;
2.
East China Normal University Library， Shanghai 200241， China

Received Date: 2012-03-01
Rev Recd Date: 2012-06-01
Publish Date: 2013-03-25

Abstract

Abstract

A stable GPR41 receptor cell line was used to screen candidate agonist from the secondary metabolites extracted from gulf seaweed aflatoxin c-f-3 in Putian Fujian. The cAMP results have shown that the No.17 compound Cyclo (L-Pro-L-Phe), belonging to cyclic dipeptide, is able to increase intracellular cAMP in a variety of cells (including G41-CHO, G12-CHO, Mock-CHO, SH-sy5y and HEK293). We have demonstrated that No.17 compound induced cAMP increase in a GPCR-independent manner. Co-adminstration of adenylate cyclase activating agent forskolin and No.17 compound can lead to a further increase in cAMP level compared with those treated with forskolin alone. These results have suggested that No.17 compound may induce a sustained elevation of cAMP levels by inhibiting cAMP hydrolysis. Since No.17 compound shared similar chemical structures with some known PDE inhibitors, which may be a potential of phosphodiesterase inhibitors. This is the first report that Cyclo(L-Pro-L-Phe) could regulate cAMP formation in a variety of cells (G41-CHO, G12-CHO, Mock-CHO, SH-sy5y and HEK293).
- secondary metabolites of marine fungi,
- cAMP,
- Cyclo(L-Pro-L-Phe),
- phosphodiesterase inhibitor

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References(1)

References

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