Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility

MA Bing; ZHAI Wan-yin; ZHANG Jia-min; SUN Xiao-ning;  ZHU Zi-yan; ZHANG Hong-feng; CHANG Jiang

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Sep. 2013

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2013 > (5): 61-70, 79

MA Bing, ZHAI Wan-yin, ZHANG Jia-min, SUN Xiao-ning,  ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 61-70, 79.

Citation:

MA Bing, ZHAI Wan-yin, ZHANG Jia-min, SUN Xiao-ning,  ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 61-70, 79.

Citation:

MA Bing, ZHAI Wan-yin, ZHANG Jia-min, SUN Xiao-ning,  ZHU Zi-yan, ZHANG Hong-feng, CHANG Jiang. Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 61-70, 79.

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Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility

1.
School of Life Sciences, East China Normal University, Shanghai 200062, China;
2.
Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;
3.
Shanghai Blood Center, Shanghai Institute of Blood Transfusion, Shanghai 200051, China;
4.
Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China

Received Date: 2012-07-01
Rev Recd Date: 2012-10-01
Publish Date: 2013-09-25

Abstract

Abstract

In this study, decellularized bovine pericardium matrix (dbpECM) was cross-linked by using PC at series concentrations. Mechanical properties, stability and hemo-compatibility of PC-crosslinked dbpECM (PC-dbpECM) were investigated. The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level. Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked. When PC-dbpECM soaked in D-Hanks solution, only the excessive non-crosslinked PC released within a week, leaving the crosslinked PC stable all the experiment period. PC crosslinked dbpECM (1 mg/mL) can effectively inhibit calcification in vitro. Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA. The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM (7.8%) was significantly lower than that of non-crosslinked group (26.1%) and the GA crosslinked group (36.6%), indicating that PC-dbpECM has low coagulation spotential. These data suggest that PC-dbpECM has good mechanical properties, stability and hemo-compatibility, suitable for preparing biological heart valves.
- procyanidins,
- decellularized bovine pericardium,
- mechanical properties,
- stability,
- hemo-compatibility

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References(1)

References

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