An exploratory study on identification means <em>Ulva linza</em> from floating green algae

CHEN Bin-bin; MA Jia-hai; SHIN Jong-Ahm; GAO Song

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2013 > (5): 71-79

CHEN Bin-bin, MA Jia-hai, SHIN Jong-Ahm, GAO Song. An exploratory study on identification means Ulva linza from floating green algae[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 71-79.

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CHEN Bin-bin, MA Jia-hai, SHIN Jong-Ahm, GAO Song. An exploratory study on identification means Ulva linza from floating green algae[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 71-79.

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An exploratory study on identification means Ulva linza from floating green algae

1.
Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China 2. Aquaculture Program, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu 550-749, Korea

Received Date: 2012-09-01
Rev Recd Date: 2012-12-01
Publish Date: 2013-09-25

Abstract

Abstract

To study on morphological characteristics of \textit{Ulva linza} from floating green algae, we selected specimens with the typical features of \textit{U. linza. }Only 4 specimens from 25 samples were determined as \textit{U. linza }from the morphological characteristics results. The molecular biology research by means of nuclear-encoded ITS sequences analysis was also carried out. Fifteen floating specimens with characters of \textit{U. linza} clustered into the LPP (\textit{U. linza-procera-prolifera}) complex group including the attached species DF05 from Jiangsu Province, QD03 from Shandong Province and XS01, XS02 from Zhejiang Province. The floating green algae from Rudong inland river and coastal pool of Sheyang were included in LPP complex as well. \textit{U. flexuosa} group included two specimens that just were fixation growth algae from Fujian Province. \textit{U. compressa} group had four specimens, and they all were floating green algae. A great disparity existed between the morphological characteristics and the molecular results. Identification of \textit{U. linza} and other floating green algae based on either morphology or ITS sequence unilaterally was not reliable. Almost all of floating green algae from the same location had certain morphological types, and they were considered to be different species by the ITS sequences analysis. The result of N-J tree and ITS data showed that the species in LPP complex and \textit{U. compressa} existed in the green tide. But\textit{ U. flexuosa }did not exist in the floating green algae in this research.
- green tide,
- Ulva linza,
- morphology,
- ITS,
- molecular biology

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