Investigation on chemiluminescence detection of vitamin B<sub>12</sub> with capillary electrophoresis

ZHU Jin-kun; SHU Lu; WU Min; WANG Qing-jiang; HE Pin-gang; FANG Yu-zhi

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Sep. 2013

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Article Navigation > Journal of East China Normal University (Natural Sciences) > 2013 > (5): 96-101

ZHU Jin-kun, SHU Lu, WU Min, WANG Qing-jiang, HE Pin-gang, FANG Yu-zhi. Investigation on chemiluminescence detection of vitamin B12 with capillary electrophoresis[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 96-101.

Citation:

ZHU Jin-kun, SHU Lu, WU Min, WANG Qing-jiang, HE Pin-gang, FANG Yu-zhi. Investigation on chemiluminescence detection of vitamin B₁₂ with capillary electrophoresis[J]. Journal of East China Normal University (Natural Sciences), 2013, (5): 96-101.

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Investigation on chemiluminescence detection of vitamin B₁₂ with capillary electrophoresis

1.
Department of Chemistry, East China Normal University, Shanghai 200241, China
2.
College of Science, Anhui Science and Technology University,Fengyang Anhui 233100, China

Received Date: 2012-06-01
Rev Recd Date: 2012-09-01
Publish Date: 2013-09-25

Abstract

Abstract

Based on the chemiluminescence (CL) catalysis of vitamin B12 (VB12 in luminol-H$_{2}$O$_{2}$ system, a novel capillary electrophoresis-chemiluminescence (CE-CL) method was developed for the accurate determination of this important nutrient substance. First of all, sodium hydrosulfite (Na$_{2}$S$_{2}$O$_{4})$ was found to be used as an competent reduction reagent to pre-reduce VB12(Co(III)) into VB12(Co(II)), which produced strong CL emission in the CL reaction of luminol-H$_{2}$O$_{2}$. In this process, some interfering CL noise was observed and capillary electrophoresis (CE) was taken to remove it by efficient separation. In this way, VB12 could be reduced gently and determined accurately by the catalyzed CL signal with our lab-constructed CE-CL system. The proposed CE-CL determination could be finished in 20 min with a detection limit (LOD) of 2$\times $10$^{-7}$ mol/L ($S$/$N$=3), a linear range from 6$\times $10$^{-7}$ to 6$\times$10$^{-4}$ mol/L ($r^{2}$=0.968) and a relative standard deviation (RSD) of 2.4{\%} ($n$=9). Utilizing this method, satisfactory results were obtained by testing VB12 tablets with a recovery of 97{\%}$\sim $106{\%}. Compared to previously reported CL analysis of VB12, we dealt with the interference components in pre-reducing solution for the first time to gain more reliable data with convenient operations.
- vitamin B12,
- chemiluminescence,
- capillary electrophoresis,
- sodium hydrosulfite

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References(1)

References

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