EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in <em>Spodoptera litura.</em>

ZHAO Jin-cheng; WU Tian-min; LIU Li-hua; WANG Yang; HE Lin

Issue 1

Sep. 2015

Turn off MathJax

Article Contents

Article Navigation > Journal of East China Normal University (Natural Sciences) > 2014 > (1): 133-142

ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.

Citation:

ZHAO Jin-cheng, WU Tian-min, LIU Li-hua, WANG Yang, HE Lin. EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.[J]. Journal of East China Normal University (Natural Sciences), 2014, (1): 133-142.

Citation:

PDF( 2182 KB)

EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.

1.
School of Life Sciences, East China Normal University, Shanghai

Received Date: 2013-01-01
Rev Recd Date: 2013-04-01
Publish Date: 2014-01-25

Abstract

Abstract

Ecdysone is the insect molting hormone which induces a series of events critical for ecdysis, beginning with the formation of a complex between ecdysteroid receptor (EcR) and its partner protein, ultraspiracle (Usp). We cloned a 600~bp partial cDNAs encoding 200 amino acids EcR protein by degenerate primers from the common cutworm, \textit{Spodoptera litura}, a destructive agricultural pest insect in East Asia. We used this fragment to design the EcR-dsRNA to interfere the corresponding gene to analysis the change in different development stages. Using the Azadirachtin (Az) feeding the RNAi injected larvae to observe the development difference with normal control. Results indicated that the EcR RNAi interference the normal ecdysis and result in incomplete pupal molt. In the Az treatment after EcR RNAi injection, the larvae stunt growth and development program were disturbed and observed abnormal proleg phenotype. We deduced that Az and EcR RNAi maybe influence the key genes and inhibit the ecdysone cascade modulate pathway, which result in the disorder of tissue remodeling in molting.
- EcR,
- RNAi,
- Azadirachtin,
- Spodoptera litura.,
- proleg phenotype

FullText(HTML)

References(1)

References

[1]

{1} YAO T P, SEGRAVES W A, ORO A E, et al. Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation [J]. Cell, 1992, 71: 63-72.
{2} FOULK M S, WAGGENER J M, JOHNSO J M, et al. Isolation and characterization of the ecdysone receptor and its heterodimeric partner ultraspiracle through development in \textit{Sciara coprophila} [J]. Chromosoma, 2013, 122(1-2):103-19.
{3} JOHNSTON D M, SEDKOV Y, PETRUK S, et al. Ecdysone- and NO-mediated gene regulation by competing EcR/Usp and E75A nuclear receptors

during Drosophila development [J]. Mol Cell, 2011, 44(1):51-61.
{4} KOELLE M R, TALBOT W S, SEGRAVES W A, et al. The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor

superfamily[J]. Cell, 1991, 67: 59-77.
{5} SHINJI NAGATA, TAKARUMI MARUYAMA, TSUYOSHI OHIRA, et al. Cloning and characterization of ecdysone peceptor and ultraspiracle cDNAs from

\textit{Spodoptera litura}[J]. Ann N Y Acad Sci, 2005, 1040: 417-419.
{6} CARTHEW R W. Gene silencing by double stranded RNA [J]. Curr Opin Cell Biol, 2001, 13: 244-248.
{7} BETTENCOURT R, TERENIUS O, FAYE I. Hemolin gene silencing by ds-RNA injected into Cecropia pupae is lethal to next generation embryos [J]. Insect Molecular Biology [J], 2002, 11(3): 267-271.
{8} TALBOT W S, SWYRYD E A, HOGNESS D S. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms [J]. Cell, 1993, 73: 1323-1337.
{9} JIANHUA H, YONG Z, MINGHUI L, et al. RNA interference-mediated silencing of the bursicon gene induces defects in wing expansion [J]. FEBS Letters, 2007, 581: 697-701.
{10} RAJAGOPAL, SIVAKUMAR S, AGRAWAL N, et al. Silencing of midgut aminopeptidase N of \textit{Spodoptera litura }by double-stranded RNA establishes its role as \textit{Bacillus thuringiensis }toxin receptor[J]. The Journal of Biological Chemistry,2002, 277(49): 46849-46851;
{11} TARLOCHAN S D, GLENN R C and DAT P L. New insecticides with ecdysteroidal and juvenile hormone activity [J]. Annu Rev Entomol,1998, 43:545-69.
{12} ALASDAIR J NISBET, A. JENNIFER MORDUE (LUNTZ), ROBERT B. GROSSMAN et al. Characterization of Azadirachtin Binding to Sf9 Nuclei In Vitro[J]. Archives of Insect Biochemistry and Physiology, 2001, 46:78-86.
{13} MANAL M.ADEL, FRANTISEK SEHNAL. Azadirachtin potentiates the action of ecdysteroid agonist RH-2485 in\textit{ Spodoptera littoralis}[J]. Journal of Insect Physiology, 2000, 46:267-274.
{14} MAREK ORLOWSKI, MONIKA SZYSZKA, AGNIESZKA KOWALSKA et al. Plasticity of the Ecdysone Receptor DNA Binding Domain[J]. Molecular Endocrinology, 2004, 18(9): 2166-2184.
{15} DEVARAKONDA S, HARP JM, KIM Y, OZYHAR A, RASTINEJAD F. Structure of the heterodimeric ecdysone receptor DNA-binding complex[J]. EMBO J, 2003, 22: 5827-5840.
{16} DAVID MARTIN, OSCAR MAESTRO, JOSEFA CRUZ et al. RNAi studies reveal a conserved role for RXR in molting in the cockroach \textit{Blattella germanica}[J]. Journal of Insect Physiology, 2006, 52(4): 410-416.
{17} S.BANERJEE, H.REMBOLD. Azadirachtin a interferes with control of serotonin pools in the Neuroendocrine System of Locusts[J]. Naturwissenschaften, 1992, 79:81-84.
{18} MANAL M. ADEL, FRANTISEK SEHNAL. Azadirachtin potentiates the action of ecdysteroid agonist RH-2485 in \textit{Spodoptera littoralis}[J]. Journal of Insect Physiology, 2000, 46: 267-274.
{19} TRUMAN, J. W. Neuroendocrine control of ecdysis [G]//OHNISHI E, ISHIZAKI H. Molting and Metamorphosis: In commemoration of the fiftieth anniversary of Soichi Fukuda's discovery of prothoracic gland function. Tokyo Berlin New York: Japan Scientific Societies Press, Springer-Verlag, 1990: 67-82.
{20} TONG-RUEI L, MICHAEL BENDER. A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila[J]. Development, 2000, 127: 2897-2905.

Relative Articles

Supplements(0)

Cited By

Proportional views