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摘要: 拟南芥URO基因与生长素的代谢密切相关,为了深入了解URO基因的作用机制及其所参与的遗传途径,以拟南芥叶发育异常的半显性突变体uro(upright rosette)为材料,利用EMS诱变和Ac/Ds转座子系统这两种方法筛选uro突变体的回复子.通过这两种方法获得了一些回复子,并对这些回复子进行了验证和初步分析,为今后的研究工作奠定了良好的基础.
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关键词:
- 拟南芥 /
- URO /
- EMS诱变 /
- Ac/Ds转座子系统
Abstract: It was suggested that URO function might be associated with auxinmediated plant development. In order to know the role and the genetic net work ofURO gene, suppressors(double mutants with suppressed phenotype of uromutant) were screened through EMS mutagenesis and Ac /Ds transposon system. Several suppressors have been selected, validated and further analyzed. These suppressors will provide important clews for the function network of URO gene.
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